WebAug 1, 2024 · Briefly, 1 × 10 6 IMR-32 cells were seeded in a 6-well plate 1 day before the experiment. The next day, cells were collected and centrifuged at 3,000 g for 5 minutes. The cells were washed with HBSS buffer and centrifuged at 3,000 g for 5 minutes. Collected cells were stained with 10 μM FeRhoNox-1 in HBSS for 30 minutes in a CO 2 incubator ... WebMar 3, 2024 · The decreases in FeRhoNox-1 staining were blocked by the two-pore antagonist NED-19 (10 µM). An ANOVA with Tukey’s post hoc multiple comparisons test was used for analysis. Each data point represents the mean fluorescence of 300 endolysosomes from ten cells performed independently from three cell-culture …
亚铁离子荧光探针FeRhoNox-1 - 西安瑞禧生物技术有限公司
WebFeb 5, 2024 · The fluorescence dye FeRhoNox-1 was specific for Fe 2+ and localized to endolysosomes in U87MG astrocytoma cells and primary rat cortical neurons; in U87MG cells the endolysosome concentration of Fe 2+ ([Fe 2+] el) was 50.4 μM in control cells, 73.6 μM in ferric ammonium citrate (FAC) treated cells, and 12.4 μM in cells treated with the … Webof Fe2+ using FeRhoNox-1 showed that 5-μM atRAL dramat-ically enhanced intracellular Fe2+ levels in 661W photore-ceptor cells at 3 to 6 h of exposure (Fig. 2, A–B). The mechanisms by which cellular iron homeostasis is modulated have been well elucidated (41, 42). Transferrin (TF) captures ferric iron (Fe3+) and binds to transferrin receptor ... se tool library portland
FeRhoNox-1 (Fe2+indicator) 亚铁离子荧光探针-生化试剂-上海觅 …
WebRhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe 2+. RhoNox-1 can generate an irreversible orange (red) … Web产品描述. 铁死亡(Ferroptosis)是一种铁依赖形式的非凋亡性细胞死亡。爱 拉斯汀(Erastin)是一种铁死亡激活剂(诱导剂)。 体外诱导铁死亡性细胞坏死,这一效应可被铁死亡抑制剂(比如:Ferrostatin-1、Trolox、环匹罗司乙醇胺、放线菌酮和β-巯基乙醇)所阻 … WebFeb 1, 2024 · 8 95 96 Figure S5. Overexpressed FTN-1 counteracts the expression of SOD-5, a ROS-97 induced superoxide dismutase, during rewarming. 98 A.The levels of ftn-1 mRNA, measured by RT-qPCR, in animals of the indicated genotypes. 99 1 day-old adults were collected at 20°C before the cold adaptation, and after one and three 100 days at … se top