2024 Psmart bac vestor

Psmart bac vestor

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August undefined, 2024

WebpSMART BAC载体质粒-BioVector NTCC保藏中心 ... Cloning vector pSMART BAC v2.0, complete sequence. GenBank: EU101022.1. WebThe CopyRight pSMART BAC cloning vector incorporates transcription-free cloning for the highest stability possible. The vector is supplied pre-cut at BamHI or HindIII sites, with dephosphorylated ends. Insert DNAs with 5’-phosphorylated ends are ligated to the CopyRight vector and transformed into BAC-Optimized Replicator v2.0 Electrocompetent …

pSMART LCKan Sequence and Map - SnapGene

WebThe pSMART-BAC vector was modified as described in Materials and Methods. The full-length genomic cDNA of PEDV was amplified by RT-PCR using seven overlapping fragments designated A to G, and was assembled by using the … WebSteps to making a Payment and getting a Receipt. Go to eMarket. Visit PBSC eMARKET. Select the payment that applies to you. Select Checkout. Submit payment. Receipts: 2 … prince in malay

CopyRight v2.0 BAC Cloning Kits

WebConditions we treat Complete care for all of your most common conditions WebCloning vector pSMART BAC v2.0 (Lucigen), no blue ones were observed, and 80% of the white colonies were proved self-ligated vectors according to the sequencing result (with … WebAug 1, 2024 · The CopyRight pSMART BAC cloning vector harboring the chloramphenicol resistance gene was introduced into the AnpeNPV genome to produce the AnpeNPV bacmid that could be propagated in Escherichia coli with stable replication. prince in live action cinderella

pSMART BAC v2.0 (HindIII linearized) Sequence and Map

CopyRight®v2.0 BAC Cloning Kit, pSMART® BAC HindIII with BAC …

WebApr 29, 2015 · The Lucigen pSMART BAC vector was modified to operate as a shuttle vector between E. coli and A. nidulans. The AMA1 DNA fragment, previously identified as an autonomously replicating DNA fragment from A. nidulans [ 22 ], was incorporated into pSMART BAC to create pSMART-BAC pyrGAMA1-4, or shuttle BAC vectors, and tested for … WebShuttle BAC vector: pSMART-BAC-S pSMART BAC-S oriT rApra attP ΦC31 int please i wishWebpSMART BAC v2.0. BAC vector with inducible copy number, suitable for cloning toxic inserts. Sequence Author: Lucigen. Open in SnapGene. Try SnapGene for Free. Download … please james with you to the store

"WebDec 9, 2024 · The pSMART BAC HindIII vector (7.6 kb) was fully digested and dephosphorylated by using HindIII restriction enzyme and calf intestinal alkaline phosphatase (CIAP) enzyme (NEB, UK) at 37 °C for 60 min. Afterwards, the partially digested oral metagenome was ligated into a pre-digested pSMART BAC vector by using Anza T4 … " - Psmart bac vestor

Psmart bac vestor

Web8:00 AM to - 5:00 PM. View All Events. Vistamar School is a private independent high school in the South Bay region of Los Angeles. WebThe new pSMART BAC vectors have a lacZ-sacB stuffer region, allowing uncut vector to be detected by blue/white screening and selected against by plating on sucrose. This feature completely eliminates uncut vector background, improving the efficiency of colony picking.

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WebpSMART LCKan Low-copy number vector with a kanamycin resistance marker for efficient blunt cloning of unstable sequences. Sequence Author: Lucigen Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:Lucigen Vectors More Plasmid Sets No matches HomePlasmidsLucigen … WebThe CopyRight® v2.0 BAC Cloning Kits from Lucigen provide complete large-insert BAC cloning and library construction projects with unprecedented accuracy, reliability and efficiency when used with the pSMART® BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells.

WebDescription General description Lucigen′s CopyRight® v2.0 BAC cloning kits with pSMART® BAC or pEZ™ BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells … WebCopyRight®v2.0 BAC Cloning Kit, pSMART® BAC HindIII with BAC-Optimized Replicator™v2.0 Electrocompetent Cells sufficient for 20 reactions; find Sigma-Aldrich-LUC420322 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich

WebVector Database Homepage pSMART® BAC v2.0 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from … WebLucigen′s CopyRight® v2.0 BAC cloning kits with pSMART® BAC or pEZ™ BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells result in unprecedented …

WebJul 31, 2024 · The CopyRight pSMART BAC cloning vector harboring the chloramphenicol resistance gene was introduced into the AnpeNPV genome to produce the AnpeNPV bacmid that could be propagated in...

WebA cloning region from the vector pSMART BAC v2.0 (Lucigen Corporation, Middleton, WI) containing the counter-selectable sacB gene and pUC19 origin of replication was prince in love with poor girlWebThe pSMART® BAC vectors incorporate transcription-free cloning to provide maximum stability. These vectors are supplied pre-cut at BamHI, EcoRI, or HindIII sites, with … prince in malaysiaWebpSmartBAC (EU101022.1) (Search Vector Database) Backbone manufacturer Lucigen Corporation Backbone size (bp) 7648 Modifications to backbone We first restriction … please jeans glitterWebrestriction mapping. Lucigen’s pSMART® or pEZ™ BAC vector is pre-cut and dephosphorylated, eliminating the need for vector preparation. CopyRight kits contain the … prince in opera crosswordWebThe pSMART-BAC vector was modified as described in Materials and Methods. The full-length genomic cDNA of PEDV was amplified by RT-PCR using seven overlapping … prince in minneapolis houseWebThese vectors incorporate the CloneSmart® transcription-free technology, which minimizes cloning bias and enables cloning of otherwise toxic genes, along with inducible copy number amplification (20-50X) for high DNA yields and a lacZ-sacB stuffer fragment that eliminates uncut vector from recombinants, resulting in zero background. prince in my hairWebin BAC cloning and library construction. ˛ e pSMART® BAC or pEZ BAC vectors incorporate the CloneSmart® transcription-free technology to minimize cloning bias and enable … prince inn bed \u0026 breakfast snpmar23